HLA-DR antibody (FITC)

Catalog No. ABIN2749149

The Mouse Monoclonal anti-HLA-DR antibody has been validated for FACS. It is suitable to detect HLA-DR in samples from Human, Dog and Non-Human Primate. There are 6+ publications available.

Quick Overview for HLA-DR antibody (FITC) (ABIN2749149)

Target: HLA-DR

Reactivity: Human, Dog, Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This HLA-DR antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Clone: L243

Product Details anti-HLA-DR Antibody (FITC)

Purpose: Anti-HLA-DR FITC

Specificity: The mouse monoclonal antibody L243 recognizes specifically an extracellular epitope on HLA-DR molecules, both peptide-loaded and empty.

Cross-Reactivity (Details): Human, Non-Human Primates, Canine (Dog)

Purification: Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: Human B lymphocytes

Isotype: IgG2a kappa

Application Details

Application Notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.

Restrictions: For Research Use only

Handling

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

References for anti-HLA-DR antibody (FITC) (ABIN2749149)

De Gassart, Camosseto, Thibodeau, Ceppi, Catalan, Pierre, Gatti: "MHC class II stabilization at the surface of human dendritic cells is the result of maturation-dependent MARCH I down-regulation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 9, pp. 3491-6, (2008) (PubMed).

Swiatek-de Lange, Rist, Stahl, Weith, Lenter: "Comment on "MHC class II expression identifies functionally distinct human regulatory T cells"." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 180, Issue 6, pp. 3625; author reply 3626, (2008) (PubMed).

Muczynski, Ekle, Coder, Anderson: "Normal human kidney HLA-DR-expressing renal microvascular endothelial cells: characterization, isolation, and regulation of MHC class II expression." in: Journal of the American Society of Nephrology : JASN, Vol. 14, Issue 5, pp. 1336-48, (2003) (PubMed).

Bouillon, El Fakhry, Girouard, Khalil, Thibodeau, Mourad: "Lipid raft-dependent and -independent signaling through HLA-DR molecules." in: The Journal of biological chemistry, Vol. 278, Issue 9, pp. 7099-107, (2003) (PubMed).

Kalka-Moll, Tzianabos, Bryant, Niemeyer, Ploegh, Kasper: "Zwitterionic polysaccharides stimulate T cells by MHC class II-dependent interactions." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 169, Issue 11, pp. 6149-53, (2002) (PubMed).

Coral, Pucillo, Leonardi, Fonsatti, Altomonte, Maio: "Triggering of HLA-DR antigens differentially modulates tumor necrosis factor alpha release by B cells at distinct stage of maturation." in: Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research, Vol. 8, Issue 5, pp. 581-8, (1997) (PubMed).

Target Details for HLA-DR

Target: HLA-DR

Alternative Name: HLA-DR

Background: HLA-DR, a member of MHC class II glycoproteins, that bind intracellularly processed peptides and present them to the Th cells, is composed of 36 kDa alpha chain and 27 kDa beta chain, both anchored in the plasma membrane. Together with other MHC II molecules HLA-DR plays a central role in the immune system.

Pathways: Human Leukocyte Antigen (HLA) in Adaptive Immune Response